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Albumin Bromcresol green method Mono Fluid

Albumin Bromcresol green method Mono Fluid
Albumin Bromcresol green method Mono Fluid is a carbohydrate-free protein, which constitutes 55-65% of total plasma protein. It maintains oncotic plasma pressure, is also involved in the transport and storage of a wide variety of ligands and is a source of endogenous amino acids. Albumin binds and solubilizes various compounds, e. g. bilirubin, calcium and long-chain fatty acids. Furthermore albumin is capable of binding toxic heavy metals ions as well as numerous pharmaceuticals, which is the reason why lower albumin concentrations in blood have a significant effect on pharmacokinetics.

Hyperalbuminemia is of little diagnostic significance except in the case of dehydration. Hypoalbuminemia occurs during many illnesses and is caused by several factors: Compromised synthesis due either to liver disease or as a consequence of reduced protein uptake, elevated catabolism due to tissue damage (severe burns) or inflammation, malabsorption of amino acids (Crohn's disease), proteinuria as a consequence of nephrotic syndrome; protein loss via the stool (neoplastic disease). in severe cases of hypoalbuminemia, the maximum albumin concentration of plasma is 2.5 g/dl. Due to the low osmotic pressure of the plasma water permeates through blood capillaries into tissue (edema). The determination of albumin allows monitoring of a controlled patient dietary supplementation and serves also as an excellent test of liver function.

Intended use
In vitro test for the quantitative determination of albumin in human serum and plasma.

Test principle
Colorimetric assay, endpoint method
At a pH value of 4.2 albumin displays a sufficiently cationic character to be able to bind with bromcresol green (BCG), an anionic dyestuff, to form a blue-green complex.
The colour intensity of the blue-green color is directly proportional to the albumin concentration and can be determined photometrically

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