Ammonia UV liquid

Ammonia UV Liquid
In 1859, Berthelot described a reaction between ammonia and an alkaline solution of phenol hypochlorite suitable for the determination of ammonia. The assay, however, proved to be subject to interferences, and several alternatives have been proposed to eliminate the problems inherent in the method.

In 1963, Kirsten, et al introduced an enzymatic method for ammonia determination based on the action of glutamate dehydrogenase. Although the enzymatic method proved to be highly specific and utilized direct evaluation based on the molar absorptivity of NADH, several problems, including difficulties in stabilizing the end reaction, were encountered.

The method presented here is based on Da Fonseca-Wollheim's modification of the Kirsten reaction. The original enzymatic method is improved by the addition of ADP to the reaction mixture, the use of NADPH in place of NADH to eliminate interference from the reaction of endogenous LDH with endogenous pyruvate, and the substitution of plasma for deproteinized supernatant.

Ammonia UV liquid measurements are used in the diagnosis and treatment of severe liver disorders such as cirrhosis, hepatitis, and Reye's Syndrome.

Intended use
For the quantitative determination of Ammonia (NH3 ) in plasma on automated clinical chemistry analyzers.

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