Bilirubin Total LS Jendrassik-Grof method

Bilirubin Total LS Jendrassik-Grof method
80 – 85 % of Vilirubin Total LS originates on degradation of hemoglobin with the other 15 – 20 % being derived from cytochrome, myoglobin and catalases. Unconjugated bilirubin, which binds to plasma albumin, is produced in the course of degradation in the reticuloendothelial system, liver Kupffer cells, spleen and bone marrow. Unconjugated (primary, indirect, water-insoluble) bilirubin is soluble in lipids and toxic. With the aid of the enzyme glucuronyl transferase, bilirubin is conjugated primarily by glucuronic acid in the microsomes of hepatic parenchymal cells. In contrast to unconjugated bilirubin, conjugated (secondary, direct) bilirubin is soluble in water and excreted via the kidneys. Bilirubin assays are suitable for evaluating the degree of severity of icteric clinical symptoms as well as for monitoring and objectively assessing these symptoms. Distinguishing between direct and indirect bilirubin is a valuable aid in the differential diagnosis of different forms of jaundice. A direct bilirubin value of < 20 % total bilirubin is an indicator of jaundice of pre-hepatic origin. This value can increase to > 50 % in hepatic and post-hepatic jaundice.

Intended use
Quantitative determination of total bilirubin in serum, heparinized plasma or EDTA plasma by the Jendrassik- Gróf method.

Test principle
Jendrassik-Gróf method

In the presence of caffeine accelerator, total bilirubin couples with sulfanilic acid to form a red azobilirubin dye, the color intensity of which is proportional to the bilirubin concentration. Determination of direct bilirubin is performed without caffeine additive

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