CK-NAC fluid 5+1 Optimized UV-Test, NAC-Activated

CK-NAC fluid 5+1 Optimized UV-Test, NAC-Activated
Creatine kinase (CK) is a dimeric enzyme occurring in four different forms: a mitochondrial isoenzyme and the cytosolic isoenzymes CKMM (muscle type), CK-BB (brain type) and CK-MB (myocardial type).
The determination of CK and CK-isoenzyme activities is utilized in the diagnosis and monitoring of myocardial infarction and myopathies such as the progressive Duchenne muscular dystrophy. Following injury to the myocardium, such as occurs with acute myocardial infarction, CK is released from the damaged myocardial cells. In early cases, a rise in the CK activity can be found just 4 hours after an infarction. The CK-activity reaches a maximum after 12–24 hours and then falls back to the normal range after 3–4 days. Myocardial damage is very likely when the total CK activity is above 190 U/l, the CK-MB activity is above 24 U/l (37°C) and the CK-MB activity fraction exceeds 6% of the total.The assay method using creatine phosphate and ADP was first described by Oliver, modified by Rosalki and further improved for optimal test conditions by Szasz. CK is rapidly inactivated by oxidation of the sulfhydryl groups in the active center. The enzyme can be reactivated by the addition of acetylcysteine (NAC). Interference by adenylate kinase is prevented by the addition of diadenosine pentaphosphate and AMP. Standardized methods for the determination of CK using the “reverse reaction” and activation by NAC were recommended by the German Society for Clinical Chemistry (DGKC) and the International Federation of Clinical Chemistry (IFCC) in1977 and 1990 respectively.

Intended use
In vitro test for the quantitative determination of creatine kinase CK) in human serum and plasma on automated clinical chemistry analyzers.

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