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Gamma-GT SZASZ-74 method

Gamma-GT SZASZ-74 method
Gamma-glutamyltransferase is used in the diagnosis and monitoring of hepatobiliary diseases, Enzymatic activity of GGT is often the only parameter with increased values when testing for such diseases, and is one of the sensitive indicators known. Gamma-glutamyltransferase is also a sensitive screening test for occult alcoholism. Elevated GGT activities are found in the serum of patients requiring long-term medication with phenobarbital and phenytoin.

In 1969, Szasz published the first kinetic procedure for GGT in serum using γ-glutamyl-p-nitroanilide as substrate and glycylglycine as acceptor. In order to circumvent the poor solubility of γ-glutamyl-p-nitroanilide, Persijn and van der Slik investigated various derivatives of the compound with respect to solubility. The substrate L-γ-glutamyl-3-carboxy-4-nitroanilide is superior in terms of stability and solubility.

The assay described below uses the water-soluble substrate L-γ-glutamyl-3-carboxy-4-nitroanilide . The results correlate with those derived using the original substrate.

Intended use
In vitro test for the quantitative determination of gamma-Glutamyl-transferase (GGT) in human serum and plasma.

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