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GLDH Fluid 4+1 IFCC

GLDH Fluid 4+1 IFCC
GLDH is a largely liver-specific enzyme found exclusively in the mitochondria and located predominantly in the azinus central liver cells. GLDH activity in other organs such as the kidneys, pancreas, heart, brain, and intestines is negligible. Determination of GLDH activity is performed to diagnose liver disorders, and in particular to assess the
severity of damage to individual cells. Nectrotizing liver damage such acute hepatic dystrophy, necrotizing hepatitis, multiple liver metastases and obstructive jaundice are accompanied by elevated GLDH activities in serum.
In 1972, the German Society for Clinical Chemistry (DGKC) recommended the optimised standard method for determination of GLDH with optimised substrate concentration, NADH excess, and activation of GLDH by addition of ADP. The current assay conforms with DGKCrecommendations.

Intended use
Assay for the quantitative in vitro determination of glutamate dehydrogenase (GLDH) in human serum and plasma on automated clinical chemistry analyzers.

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