LDH-L IFCC Fluid 5+1

LDH-L IFCC Fluid 5+1
Lactate Dehydrogenase measurements are used in the diagnosis and treatment of liver diseases such as acute viral hepatitis, cirrhosis, and metastatic carcinoma of the liver, cardiac diseases such as myocardial infarction, and tumours of the lungs or kidneys. In 1956, Wacker et al1
published a method for LD determination using lactate as substrate, buffered at pH 8.8, with NAD as coenzyme. In 1968, Gay et al2 presented a method "optimising" the Wacker procedure with respect to pH, substrate, and coenzyme concentrations. The procedure presented here represents a further modification of the Gay procedure
in that tris-(hydroxymethyl)-aminomethane (Tris) is used as
the buffer.3

Intended use
For the quantitative determination of lactate dehydrogenase in human serum and plasma on automated clinical chemistry analyzers.

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