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Lipase Color Fluid

Lipase Color Fluid
Lipases are glycoproteins with a molecular weight of 47000 daltons.

They are defined as triglyceride hydrolases which catalyze the cleanse of triglycerides to diglycerides with subsequent formation of monoglycerides and fatty acids. In addition to a-amylase, pancreatic lipases have for many years been undeniably the most important clinical chemistry parameters for the differential diagnosis of diseases of the pancreas. The lipase activity determination has gained increasing international recognition because of its high specificity and rapid response. After acute pancreatitis the lipase activity increases within 4-8 hours, reaches a peak after 24 hours and decreases after 8 to 14 days. However, there is no correlation between the lipase activity determined in serum and the extent of damage to the pancreas. Numerous methods have been described for the determination of lipase which determine the decrease in substrate turbidimetrically or nephelometrically or determine degradation products.

This method is based on the cleavage of a specific chromogenic lipase substrate 1,2-O-dilauryl-rac-glycero-3-glutaric acid-(6-methylresorufin) ester emulsified with bile acids. The pancreatic enzyme activity is determined specifically by the combination of bile acid and colipase used in this assay. Virtually no lipase activity is detected in the absence of colipase. Colipase only activates pancreatic lipase, but not other lipolytic enzymes found in serum. The high amount of cholates ensure that the esterases present in the serum do not react with the chromogenic substrate due to the highly negative surface charge.

Intended use
Enzymatic assay for the in vitro quantitative determination of Lipase in human serum and plasma.

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