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Phosphate UV Molybdate-UV Mono Fluid

Phosphate UV Molybdate-UV Mono Fluid
88% of the phosphorous contained in the body is localized in bone in the form of calcium phosphate as the apatite Ca2+[Ca3 (PO)]2-3. The remainder is involved in intermediary carbohydrate metabolism and in physiologically important substances such as phospholipids, nucleic acids and ATP Phosphorus occurs in blood in the form of inorganic phosphate and in organically bound phosphoric acid. The small amount of extracellular organic phosphorus is found almost exclusively in the form of phospholipids.

The ratio of phosphate to calcium in the blood is approximately 6:10. An increase in the level of phosphorus causes a decrease in the calcium level. The mechanism is influenced by interactions between parahormone and vitamin D. Hypoparathyroidism, vitamin D intoxication and renal failure with decreased glomerular phosphate filtration give rise to hyperphosphatemia. Hypophosphatemia occurs in rickets, hyperparathyroidism and Fanconi's syndrome.

The preferred method for the determination of inorganic phosphorus is based on the formation of ammonium phosphomolybdate with subsequent reduction to molybdenum blue. Reagent stability problems often occur with this method. The method presented here is based on the reaction of phosphate with ammonium molybdate to form ammonium phosphomolybdate without reduction. The addition of an accelerator gives rise to a more rapid rate of reaction and the application of sample blanking yields more precise results.

Intended use
In vitro test for the quantitative determination of phosphorus in human serum, plasma and urine

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