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Uric Acid Mono

Uric Acid Mono
Uric acid is the final product of purine metabolism in the human organism. Uric acid measurements are used in the diagnosis and treatment of numerous renal and metabolic disorders, including renal failure, gout, leukaemia, psoriasis, starvation or other wasting conditions, and of patients receiving cytotoxic drugs.

The oxidation of uric acid provides for two approaches to the quantitative determination of this purine metabolite. One approach is the reduction of phosphotungstic acid in an alkaline solution to tungsten blue, which is measured photometrically. The method is, however, subject to interferences from drugs and reducing substances other than uric acid.

A second approach, described by Praetorius and Poulson, utilizes the enzyme uricase to oxidise uric acid; this method eliminates the interferences intrinsic to chemical oxidation. Uricase can be employed in methods that involve the UV measurement of the consumption of uric acid or in combination with other enzyme to provide a colorimetric method.

The assay described here is a slight modification of the colorimetric method. The modifications were described by Siedel. This reaction, the peroxide reacts in the presence of peroxidase, DHBSA and aminoantipyrine to form a quinoneimine dye. The intensity of the red color is proportional to the uric acid concentration and is determined photometrically.

Intended use
Enzymatic in vitro test for the quantitative determination of uric acid in human serum and plasma.

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